Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying ou...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-02, Vol.51 (12), p.2283-2285
Hauptverfasser: Carlin, D A, Bertolani, S J, Siegel, J B
Format: Artikel
Sprache:eng
Schlagworte:
Binding Sites
Biocatalysis
Ethylene Oxide - chemistry
Ethylene Oxide - metabolism
Ethylenes - chemistry
Ethylenes - metabolism
Molecular Dynamics Simulation
Oxidation-Reduction
Oxygenases - genetics
Oxygenases - metabolism
Protein Structure, Tertiary
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08802f