Explore protein molecular structure in endosperm tissues in newly developed black and yellow type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy

•Yellow canola seeds contained relatively higher percentage of a-helix and β-sheet than black canola seeds.•Yellow canola seeds contained higher percentage of amide I and amide II area compared to the black canola seed.•Not significant molecular structural differences in protein amide I and II region between yellow and black canola seed. This study was conducted to characterize the protein molecular structure in endosperm tissues in newly developed black and yellow-type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy. The results showed that the yellow canola seeds contained relatively lower (P