Preservation of viable Francisella tularensis for forensic analysis

As a preservation solution, (1%) ammonium chloride may be preferred over other conventionally used storage solutions because of its compatibility with analytical techniques such as Mass Spectrometry. In this study, ammonium chloride performed as well or better than phosphate buffered saline with Twe...

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Veröffentlicht in:	Journal of microbiological methods 2011-02, Vol.84 (2), p.346-348
Hauptverfasser:	Valentine, Nancy B., Wunschel, Sharon C., Valdez, Catherine O., Kreuzer, Helen, Bartholomew, Rachel A., Straub, Timothy M., Wahl, Karen L.
Format:	Artikel
Sprache:	eng
Schlagworte:	ammonium chloride Ammonium Chloride - metabolism AMMONIUM CHLORIDES Bacteriological methods and techniques used in bacteriology Bacteriology BASIC BIOLOGICAL SCIENCES Biological and medical sciences COMPATIBILITY Forensic Medicine - methods forensic sciences Francisella Francisella tularensis Francisella tularensis - isolation & purification Francisella tularensis subsp. novicida Fundamental and applied biological sciences. Psychology GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE Humans mass spectrometry MASS SPECTROSCOPY Microbial Viability Microbiology PHOSPHATES PRESERVATION Preservation, Biological - methods Specimen Handling - methods STORAGE
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Zusammenfassung:	As a preservation solution, (1%) ammonium chloride may be preferred over other conventionally used storage solutions because of its compatibility with analytical techniques such as Mass Spectrometry. In this study, ammonium chloride performed as well or better than phosphate buffered saline with Tween or Butterfields/Tween for preserving Francisella tularensis subsp. novicida.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2010.12.006