Inhibition of Tyrosine Hydroxylase by (1R,9S)-β-Hydrastine Hydrochloride in PC12 cells

It is reported that (1R,9S)-β-Hydrastine hydrochloride (BHSH) decreased the intracellular dopamine content by inhibiting tyrosine hydroxylase (TH) activity in PC12 cells. In this study, the inhibitory mechanisms on TH activity by BHSH in PC12 cells were investigated. BHSH treatment caused a reductio...

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Veröffentlicht in:	Natural product sciences 2004-06, Vol.10 (3), p.114-118
Hauptverfasser:	Yin, Shou Yu (Yanbian University, Yanji, Jilin, P.R. China), Kim, Y.M. (Chungbuk National University, Cheongju, Republic of Korea), Lee, J.J. (Chungbuk National University, Cheongju, Republic of Korea), Jin, C.M. (Chungbuk National University, Cheongju, Republic of Korea), Yang, Y.J. (Chungbuk National University, Cheongju, Republic of Korea), Lim, K.W. (Chungbuk National University, Cheongju, Republic of Korea), Kang, M.H. (Chungbuk National University, Cheongju, Republic of Korea), Lee, M.K. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: myklee@chungbuk.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	(1R,9S)-β-Hydrastine hydrochloride [Ca²+]∧i CELLS CELLULE CELULAS Cyclic AMP PC12 cells TIROSINA TYROSINE Tyrosine hydroxylase
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Zusammenfassung:	It is reported that (1R,9S)-β-Hydrastine hydrochloride (BHSH) decreased the intracellular dopamine content by inhibiting tyrosine hydroxylase (TH) activity in PC12 cells. In this study, the inhibitory mechanisms on TH activity by BHSH in PC12 cells were investigated. BHSH treatment caused a reduction of TH activity and TH mRNA level in a dose-dependent manner. After the treatment of 20 μM BHSH, TH activity and TH mRNA content were reduced at 15min, reached the minimal levels at 6-24h, and then recovered gradually to the control level.
ISSN:	1226-3907 2288-9027