Development of Pretreatment Method for Analysis of Vitamin B12 in Cereal Infant Formula using Immunoaffinity Chromatography and High-Performance Liquid Chromatography

Vitamin B 12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC)...

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Veröffentlicht in:Food science of animal resources 2021, 41(2), , pp.335-342
Hauptverfasser: Park, Jung Min, Koh, Jong Ho, Kim, Jin Man
Format: Artikel
Sprache:eng
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Zusammenfassung:Vitamin B 12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC) was developed for the rapid detection and quantitation of vitamin B 12 in powdered milk. Detection limit and powdered milk recovery were determined by quantitative analysis. The limits of detection and quantitation were 2.71 and 8.21 μg/L, respectively. Relative standard deviations of the intra-day and inter-day precisions varied in the ranges of 0.98%–5.31% and 2.16%–3.90%, respectively. Recovery of the analysis varied in the range of 83.41%–106.57%, suggesting that the values were acceptable. Additionally, vitamin B 12 content and recovery in SRM 1849a were 54.10 μg/kg and 112.24%, respectively. Our results suggested that the analytical method, including the sample pretreatment step, was valid. This analytical method can be implemented in many laboratory-scale experiments that seek to save time and labor. Therefore, this study shows that immunoaffinity–HPLC/ultraviolet is an acceptable technique for constructing a reliable database on vitamin B 12 in powdered milk containing starch as well as protein and/or fat in high amounts.
ISSN:2636-0772
2636-0780
DOI:10.5851/kosfa.2020.e92