Development of Pretreatment Method for Analysis of Vitamin B12 in Cereal Infant Formula using Immunoaffinity Chromatography and High-Performance Liquid Chromatography
Vitamin B 12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC)...
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Veröffentlicht in: | Food science of animal resources 2021, 41(2), , pp.335-342 |
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Sprache: | eng |
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Zusammenfassung: | Vitamin B
12
deficiency may lead to serious health issues in both
infants and adults. A simple analytical method involving sample pretreatment
with enzyme, followed by cyanide addition under acidic conditions; separation on
an immunoaffinity column; and high-performance liquid chromatography (HPLC) was
developed for the rapid detection and quantitation of vitamin B
12
in
powdered milk. Detection limit and powdered milk recovery were determined by
quantitative analysis. The limits of detection and quantitation were 2.71 and
8.21 μg/L, respectively. Relative standard deviations of the intra-day
and inter-day precisions varied in the ranges of
0.98%–5.31% and 2.16%–3.90%,
respectively. Recovery of the analysis varied in the range of
83.41%–106.57%, suggesting that the values were acceptable.
Additionally, vitamin B
12
content and recovery in SRM 1849a were
54.10 μg/kg and 112.24%, respectively. Our results suggested that
the analytical method, including the sample pretreatment step, was valid. This
analytical method can be implemented in many laboratory-scale experiments that
seek to save time and labor. Therefore, this study shows that
immunoaffinity–HPLC/ultraviolet is an acceptable technique for
constructing a reliable database on vitamin B
12
in powdered milk
containing starch as well as protein and/or fat in high amounts. |
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ISSN: | 2636-0772 2636-0780 |
DOI: | 10.5851/kosfa.2020.e92 |