Monthly distribution of ammonia-oxidizing microbes in a tropical bay
Ammonia oxidation, performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB), plays a critical role in the cycle of nitrogen in the ocean. For now, environmental variables controlling distribution of ammonia-oxidizing microbes are still largely unknown in oceanic environments. In this study, w...
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Veröffentlicht in: | The journal of microbiology 2021, 59(1), , pp.10-19 |
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Sprache: | eng |
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Zusammenfassung: | Ammonia oxidation, performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB), plays a critical role in the cycle of nitrogen in the ocean. For now, environmental variables controlling distribution of ammonia-oxidizing microbes are still largely unknown in oceanic environments. In this study, we used real-time quantitative PCR and high-throughput sequencing methods to investigate the abundance and diversity of AOA and AOB from sediment and water in Zhanjiang Bay. Phylogenic analysis revealed that the majority of AOA
amoA
sequences in water and sediment were affiliated with the genus
Nitrosopumilus
, whereas the
Nitrosotalea
cluster was only detected with low abundance in water.
Nitrosomonas
and
Nitrosospira
dominated AOB
amoA
sequences in water and sediment, respectively. The
amoA
copy numbers of both AOA and AOB varied significantly with month for both sediment and water. When water and sediment temperature dropped to 17–20°C in December and February, respectively, the copy number of AOB
amoA
genes increased markedly and was much higher than for AOA
amoA
genes. Also, AOA abundance in water peaked in December when water temperature was lowest (17–20°C). Stepwise multiple regression analyses revealed that temperature was the most key factor driving monthly changes of AOA or AOB abundance. It is inferred that low water temperature may inhibit growth of phytoplankton and other microbes and so reduce competition for a common substrate, ammonium. |
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ISSN: | 1225-8873 1976-3794 |
DOI: | 10.1007/s12275-021-0287-5 |