Analysis of Plant Metabolites Damaged in Chemical Accidents Using NMR Spectroscopy

Chemical accidents can cause a variety of damage, including damage to human health and ecosystems. In Korea, there are a limited number of crops that can be used to calculate damage done to plants after chemical accidents, and there are insufficient data on testing of damaged plants and methods for evaluation thereof because damage calculation methods and procedures have not been specifically established. Therefore, in order to estimate economic losses due to chemical accidents, it is necessary to establish specific calculation methods and procedures. At this point, improvements in Nuclear Magnetic Resonance (NMR) spectroscopy are needed to improve the accuracy and precision of assessment of plant damage caused by chemical accidents. The purpose of this study is to standardize sample pretreatment methods for NMR application and to develop a methodology for estimation of plant damage via testing and evaluation methods for solid samples such as plants and powders at chemical accident sites. 1H solution‐state NMR spectra of aqueous extract of oak leaves. Oak leaves exposed to 5% HCl, the peaks in the aromatic region markedly increased and structural changes in amino acids have occurred and oak leaves exposed to 5% NH3 differed from control leaves in the region where the amino acid peaks appeared. Region A: aromatic region. Region B: sugars & derivatives. Region C: typically, amino acids.