Impact of small RNA RaoN on nitrosative-oxidative stress resistance and virulence of Salmonella enterica serovar Typhimurium
RaoN is a Salmonella -specific small RNA that is encoded in the cspH - envE intergenic region on Salmonella pathogenicity island-11. We previously reported that RaoN is induced under conditions of acid and oxidative stress combined with nutrient limitation, contributing to the intramacrophage growth...
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Veröffentlicht in: | The journal of microbiology 2020, 58(6), , pp.499-506 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | RaoN is a
Salmonella
-specific small RNA that is encoded in the
cspH
-
envE
intergenic region on
Salmonella
pathogenicity island-11. We previously reported that RaoN is induced under conditions of acid and oxidative stress combined with nutrient limitation, contributing to the intramacrophage growth of
Salmonella enterica
serovar Typhimurium. However, the role of RaoN in nitrosative stress response and virulence has not yet been elucidated. Here we show that the
raoN
mutant strain has increased susceptibility to nitrosative stress by using a nitric oxide generating acidified nitrite. Extending previous research on the role of RaoN in oxidative stress resistance, we found that NADPH oxidase inhibition restores the growth of the
raoN
mutant in LPS-treated J774A.1 macrophages. Flow cytometry analysis further revealed that the inactivation of
raoN
leads to an increase in the intracellular level of reactive oxygen species (ROS) in
Salmonella
-infected macrophages, suggesting that RaoN is involved in the inhibition of NADPH oxidase-mediated ROS production by mechanisms not yet resolved. Moreover, we evaluated the effect of
raoN
mutation on the virulence in murine systemic infection and determined that the
raoN
mutant is less virulent than the wild-type strain following oral inoculation. In conclusion, small regulatory RNA RaoN controls nitrosative-oxidative stress resistance and is required for virulence of
Salmonella
in mice. |
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ISSN: | 1225-8873 1976-3794 |
DOI: | 10.1007/s12275-020-0027-2 |