Influence of load on the recycling stability of nanospheres attached platinium ion for determination of glucose

New nanospheres have been prepared with Pt2+ and Pt4+ for compare enzymatic properties of glucose oxidase enzyme (GOx). In this study nanoparticles, (aminomethyl)polystyrene (APS), 2-hydroxy-5-methylbenzaldehyde and Pt2+/Pt4+ have been synthesized by means of template and investigated the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on there. The characteristics of the immobilized glucose oxidase (APS–Pt2+–GOx and APS–Pt4+–GOx) enzyme showed one optimum pH value. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme have been investigated. It is found that nanosphere including platinium atom exhibits excellent performance as the immobilized supporter of GOx, the immobilized enzyme demonstrates perfected storage and recycling stability. APS–Pt2+–GOx retains more than 30% of the initial activity after 32 successive cycles, which is a remarkable result.