Effect of partially purified sub-fractions of Pterocarpus mildbraedii extract on carbon tetrachloride intoxicated rats

This study was designed to determine the hepatoprotective effect of partially purified fractions from Pterocarpus mildbraedii extract on carbon tetrachloride (CCl4) intoxicated rats. The methanol extract of P. mildbraedii was subjected to solvent partitioning using n-hexane, chloroform, ethylacetate and water. Separation of fractions with proven antioxidant activity was achieved by chromatographic techniques. Acute toxicity and hepatoprotective studies of the methanol sub-fraction 6 (Me 6), methanol sub-fraction 7 (Me 7) and methanol sub-fraction 8 (Me 8) from P. mildbraedii extract on carbon tetrachloride (CCl4) intoxicated Wister rats. Intoxication of rats with CCl4 resulted in significant (p0.05) in the activities of the liver enzymes, MDA level, total and direct bilirubin in dose-dependent manner. There were significant (p