Optimization of Direct Somatic Embryogenesis from Mature Zygotic Embryos of Panax ginseng C. A. Meyer

Optimization of Direct Somatic Embryogenesis from Mature Zygotic Embryos of Panax ginseng C. A. Meyer

Culture conditions were optimized for somatic embryogenesis of Panax ginseng. The highest frequency of embryo formation was obtained when tissues were excised from the middle region of the cotyledon segments of zygotic embryos. Only treatment with light could stimulate the formation of single-type s...

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Veröffentlicht in:Journal of plant biology = Singmul Hakhoe chi 2006, 49(5), , pp.348-352
Hauptverfasser: Kim, O.T. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Kim, T.S. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), In, D.S. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Bang, K.H. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Kim, Y.C. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Choi, Y.E. (Kangwon National University, Chuncheon, Republic of Korea), Cha, S.W. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), Seong, N.S. (National Institute of Crop Science, RDA, Suwon, Republic of Korea), E-mail: nsseong@rda.go.kr
Format: Artikel
Sprache:eng
Schlagworte:
AGAR
AGAR-AGAR
EMBRIOGENESIS SOMATICA
EMBRYOGENESE SOMATIQUE
GELOSE
macro salts
PANAX PSEUDOGINSENG
SACCHAROSE
SOMATIC EMBRYOGENESIS
SUCROSA
SUCROSE
생물학
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Zusammenfassung:Culture conditions were optimized for somatic embryogenesis of Panax ginseng. The highest frequency of embryo formation was obtained when tissues were excised from the middle region of the cotyledon segments of zygotic embryos. Only treatment with light could stimulate the formation of single-type somatic embryos, whereas multiple-type somatic embryos and calli were observed under dark conditions. The highest production of somatic embryos was found with an NH₄+:NO₃- ratio of 21:39.
ISSN:1226-9239
1867-0725
DOI:10.1007/bf03178810