Detection of Early Intermediates of the Glycosylphosphatidylinositol anchor in Liquid-cultured Arabidopsis

Detection of Early Intermediates of the Glycosylphosphatidylinositol anchor in Liquid-cultured Arabidopsis

Tissue extracts were prepared from liquid-culturedArabidopsis and reacted with UDP-[3H]-GlcNAc. Phospholipidfractions were then extracted by butanol partitioning. Consecutivethin-layer chromatography identified two glycolipids sensitive toPI-specific phospholipase C, known as early intermediates in...

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Veröffentlicht in:Journal of applied biological chemistry 2015, 58(1), , pp.9-11
Hauptverfasser: Cheong, Jong-Joo, Kwon, Hawk-Bin
Format: Artikel
Sprache:eng
Schlagworte:
농학
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Zusammenfassung:Tissue extracts were prepared from liquid-culturedArabidopsis and reacted with UDP-[3H]-GlcNAc. Phospholipidfractions were then extracted by butanol partitioning. Consecutivethin-layer chromatography identified two glycolipids sensitive toPI-specific phospholipase C, known as early intermediates in glycosylphosphatidylinositolanchor biosynthesis; phosphatidylinositolN-acetylglucosamine and phosphatidylinositol glucosamine. KCI Citation Count: 0
ISSN:1976-0442
2234-7941
DOI:10.3839/jabc.2015.003