산복사나무(Prunus davidiana) 열매로부터 Triterpenoid의 분리 및 동정

산복사나무(Prunus davidiana) 열매를 실온에서 80% MeOH수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, 물 분획으로 나누었다. EtOAc 분획에 대하여 SiO2과 ODScolumn chromatography를 반복 실시하여 3종의 화합물을 분리 및 정제하였다. NMR, MS, 및 IR data 등을 해석하여, ursolicacid (1), corosolic acid (2), 그리고 α-amyrin (3)로 구조동정하였다. The fruits of Prunus davidiana were extracted with 80 % aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-butyl alcohol, and water fractions. From the EtOAc fraction, three triterpenoids were isolated through the repeated silica gel (SiO2) and octadecyl SiO2 column chromatographies. Based on physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the compounds were identified to be ursolic acid (1), corosolic acid (2), and α-amyrin (3).