Enhancement of γ-Amminobutyric Acid Production by Lactobacillus sakei B2-16 Expressing Glutamate Decarboxylase from Lactobacillus plantarum ATCC 14917

To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L....

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied biological chemistry 2010, 53(6), , pp.816-820
Hauptverfasser:	Kook, M.C., Anyang University, Incheon, Republic of Korea, Seo, M.J., Brown University, Providence, RI, U.S.A, Cheigh, Chan-Ick, Ewha Womans University, Seoul, Republic of Korea, Lee, S.J., Yonsei University, Seoul, Republic of Korea, Pyun, Y.R., Yonsei University, Seoul, Republic of Korea, Park, H., Sunmoon University, Asan, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	Acid production Applied Microbiology Bacteria Biological Techniques Bioorganic Chemistry Chemistry Chemistry and Materials Science E coli gamma-amminobutyric acid Genetic engineering GLUTAMATE DECARBOXYLASE GLUTAMATO DECARBOXILASA Lactic acid Lactic acid bacteria Lactobacilli LACTOBACILLUS PLANTARUM Lactobacillus sakei Short Communication γ-Aminobutyric acid 농학
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.
ISSN:	1738-2203 2468-0834 2234-344X 2468-0842
DOI:	10.3839/jksabc.2010.123