Gene Inactivation Study on gntK, a Putative C-methyltransferase Gene in Gentamicin Biosynthesis from Micromonospora echinospora

GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on t...

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Veröffentlicht in:	Applied biological chemistry 2012, 55(3), , pp.439-442
Hauptverfasser:	Karki, Suman, Myongji University, Yongin, Republic of Korea, Kim, J.Y., Myongji University, Yongin, Republic of Korea, Park, S.H., Mokpo National University, Muan, Republic of Korea, Kwon, H.J., Myongji University, Yongin, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosylmethionine Applied Microbiology Biological Techniques Bioorganic Chemistry Biosynthesis Chemistry Chemistry and Materials Science Deactivation Domains GENE GENES Gentamicin gentamicin biosynthesis gntK Inactivation Isomers Methylation Methyltransferase Micromonospora echinospora S-Adenosylmethionine Short Communication Vitamin B12 농학
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Zusammenfassung:	GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on these results, we propose that GntK is involved in Cmethylation on C-6' in gentamicin X2 but is dispensable in gentamicin biosynthesis.
ISSN:	1738-2203 2468-0834 2234-344X 2468-0842
DOI:	10.1007/s13765-012-2041-5