Quadruple 9-mer-based Protein Binding Microarray Analysis of the Arabidopsis Transcription Factor AtMYB77

Binding sequence specificity of the Arabidopsis transcription factor AtMYB77 was determined by the quadruple 9-mer-based protein binding microarray (Q9-PBM) analysis. The position weight matrix and Wilcoxon-Mann-Whitney test with total 1843 clustered signals revealed that the full size AtMYB77 protein binds specifically to the consensus sequence pAACnG, where p represents T or C; n, A, G, C or T. This sequence is known as the type Ⅰ binding site for MYB transcription factors. This result indicates that functional diversity among the type Ⅰ MYB transcription factors is not due to their binding specificity on a particular promoter sequence.