Cosmeceutical bioactivities of isolated compounds from Ligularia fischeri Turcz leaves

Isolated compounds from the leaves of Ligularia fischeri Turcz were evaluated for their cosmeceutical bioactivities. Four novel compounds, quercetin-3- O -β- d -glucoside (LF-4), quercetin-3- O -rutinoside (LF-5), ethyl-β- d -galactopyranoside (LF-7), and pterodontriol-6- O -β- d -glucopyranoside (LF-8), were isolated for the first time from this plant. Additionally, the four known compounds, 3- O -caffeoylquinic acid (LF-1), 5- O -caffeoylquinic acid (LF-2), 3, 5-di- O -caffeoylquinic acid (LF-3), and quercetin-3- O -galactoside (LF-6), were also isolated. To demonstrate the value of this plant as a cosmeceutical resource, the antioxidant activity and the inhibitory activity on tyrosinase and elastase were evaluated. The present study is the first time that the elastase inhibition of isolated compounds from L. fischeri Turcz has been investigated using the elastase inhibitory assay for anti-wrinkle effect. The ethyl acetate fraction had the highest activities in all the evaluated assays compared with the other fractions. At the compound level, LF-3 had the greatest antioxidant and elastase inhibition effect, and LF-2 showed a high level of tyrosinase inhibition. In addition, quantitative analysis of the fractions showed that the ethyl acetate fraction had the highest amount of isolated cosmeceutical bioactive compounds: LF-2, 0.9 %; LF-3, 5.3 %; LF-5, 2.8 %; and LF-6, 12.8 %.