Profiling of skin anti-aging related proteins in human dermal fibroblasts by decursin in Angelica gigas Nakai

The extract of Angelica gigas Nakai by supercritical CO 2 increased the expression of collagen synthesis-related proteins in human dermal fibroblast, including type 1( α -2) collagen chain precursor (pI 9.08, MW 129.7), procollagen C-endopeptidase (pI 7.40, MW 47.97), and prolyl 4-hydroxylase (pI 5.49, MW 60.90). It also increased the expression level of interaction-related proteins, α -actinin (pI 5.47, MW 105.5), integrin- β 1 (pI 5.27, MW 88.46). The expression levels of these proteins by pure decursin were similar to those by supercritical fluid extract. By the dose concentration experiment, decursin in A. gigas was found to play the major role in expression level increases. Proteome analysis proved that decursin in A. gigas promoted synthesis of proteins related to skin anti-aging in human dermal fibroblasts.