Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation

Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation

A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The...

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Veröffentlicht in:Journal of medicinal food 2002, 5(4), , pp.229-234
Hauptverfasser: Atanasov, Atanas Todorov, Tchorbanov, Bojidar
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Diphosphate
Chromatography, Gel
Chromatography, Ion Exchange
Collagen
DEAE-Cellulose
Galega - chemistry
Humans
Molecular Weight
Plant Extracts - pharmacology
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - pharmacology
Thrombin
식품과학
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Zusammenfassung:A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The fraction inhibits platelet aggregation initiated by 25 microM adenosine 5'-diphosphate (ADP), 100 microg/ml collagen, and 0.8 U/ml thrombin with the 50% inhibiting concentration (IC(50)) being 11.2 microg/ml for ADP, and the IC(100) being 15.1 microg/ml for collagen and IC(100) 19.6 microg/ml for thrombin.
ISSN:1096-620X
1557-7600
DOI:10.1089/109662002763003384