Construction of a Vector Generating both siRNA and a Fluorescent Reporter: a siRNA Study in Cultured Neurons

RNA interference is an important tool for gene silencing. However, its application to primary cultured cells has been limited by low transfection efficiencies. In this work we developed a vector which encodes both siRNA and red fluorescent protein.

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Bibliographische Detailangaben
Veröffentlicht in:	Molecules and cells 2004, 18(1), , pp.127-130
Hauptverfasser:	Yoon, S.Y, Choi, J.E, Hwang, O.Y, Hong, H.N, Lee, H.R, Cho, S.W, Kim, H, Kim, D.H. (Universtiy of Ulsan College of Medicine, Seoul, Republic of Korea), Kim, Y.K. (Korea University, Seoul, Republic of Korea)
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Regulatory Proteins Bcl-2-Like Protein 11 Carrier Proteins - genetics Carrier Proteins - metabolism CELLS Cells, Cultured Doublecortin Domain Proteins Genes, Reporter Genetic Vectors - genetics Genetic Vectors - metabolism Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Humans Membrane Proteins - genetics Membrane Proteins - metabolism Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Neurons - cytology Neurons - physiology Neuropeptides - genetics Neuropeptides - metabolism Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism 생물학
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Beschreibung
Zusammenfassung:	RNA interference is an important tool for gene silencing. However, its application to primary cultured cells has been limited by low transfection efficiencies. In this work we developed a vector which encodes both siRNA and red fluorescent protein.
ISSN:	1016-8478 0219-1032
DOI:	10.1016/S1016-8478(23)13091-3