Establishment and Characterization of Three Immortal Bovine Muscular Epithelial Cell Lines

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each o...

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Veröffentlicht in:	Molecules and cells 2006, 21(1), , pp.29-33
Hauptverfasser:	Jin, Xun (Korea University, Seoul, Republic of Korea), Lee, J.S. (Korea University, Seoul, Republic of Korea), Kwak, S.W. (Korea University, Seoul, Republic of Korea), Lee, S.Y. (Korea University, Seoul, Republic of Korea), Jung, J.E. (Korea University, Seoul, Republic of Korea), Kim, T.K. (Korea University, Seoul, Republic of Korea), Xu, Chenxiong (Seoul National University, Seoul, Republic of Korea), Hong, Zhongshan (Seoul National University, Seoul, Republic of Korea), Li, Zhehu (Seoul National University, Seoul, Republic of Korea), Kim, S.M. (Seoul National University, Seoul, Republic of Korea), Pian, Xumin (Korea University, Seoul, Republic of Korea), Lee, D.H. (University of Seoul, Seoul, Republic of Korea), Yoon, J.T. (Hankyong National University, Ansung, Republic of Korea), You, S.K. (Korea University, Seoul, Republic of Korea), Choi, Y.J. (Seoul National University, Seoul, Republic of Korea), Kim, H.G. (Korea University, Seoul, Republic of Korea), E-mail: hg-kim@korea.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cell Adhesion Cell Line Cell Line, Transformed Cyclin-Dependent Kinase Inhibitor p16 - metabolism Epithelial Cell Epithelial Cells - cytology Epithelial Cells - metabolism Immortalization Kinetics MUSCLE MUSCLES Muscles - cytology MUSCULOS SV40LT Telomerase Tumor Suppressor Protein p53 - metabolism 생물학
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Zusammenfassung:	We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM.
ISSN:	1016-8478 0219-1032
DOI:	10.1016/s1016-8478(23)12899-8