Identification of Proteins Differentially Expressed in Gastric Cancer Cells with High Metastatic Potential for Invasion to Lymph Nodes

In a search for proteins involved in cancer metastasis, we analyzed proteomes of the human gastric cancer cell OCUM-2M and its metastatic subline OCUM-2MLN. We observed that aspartate aminotransferase (AAT), D-site binding protein (DBP), and anterior gradient protein 2 (AGR2) are differentially expr...

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Veröffentlicht in:	Molecules and cells 2011, 31(6), , pp.563-571
Hauptverfasser:	Lee, D.H., Seoul Women's University, Seoul, Republic of Korea, Lee, Y.R., Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Ryu, J.H., Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Park, S.G., Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Cho, S.Y., Chung-Ang University, Seoul, Republic of Korea, Lee, J.J., Chonnam National University Medical School, Gwangju, Republic of Korea, Choi, C., Chonnam National University Medical School, Gwangju, Republic of Korea, Park, B.C., Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	AAT Adenocarcinoma, Scirrhous - metabolism Adenocarcinoma, Scirrhous - pathology Adult Aged Aged, 80 and over AGR2 Amino Acid Sequence Aspartate Aminotransferases - metabolism Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Cell Biology Cell Line, Tumor DBP DNA-Binding Proteins - metabolism Electrophoresis, Gel, Two-Dimensional Female gastric cancer Humans Life Sciences Lymphatic Metastasis Male metastasis Middle Aged Mitochondrial Proton-Translocating ATPases - metabolism Molecular Sequence Data Mucoproteins Neoplasm Invasiveness NEOPLASMAS NEOPLASME NEOPLASMS Oncogene Proteins Proteins - metabolism proteome Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Transcription Factors - metabolism 생물학
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Zusammenfassung:	In a search for proteins involved in cancer metastasis, we analyzed proteomes of the human gastric cancer cell OCUM-2M and its metastatic subline OCUM-2MLN. We observed that aspartate aminotransferase (AAT), D-site binding protein (DBP), and anterior gradient protein 2 (AGR2) are differentially expressed in metastatic OCUM-2MLN cells. Measurement of protein expression in clinical samples indicated that DBP and AAT are also down-regulated in metastatic adenocarcinoma. Additionally, urokinase-type tissue plasminogen activator is up-regulated in OCUM-2MLN cells and also in metastatic gastric cancer samples. Collectively, these results raise a possibility that AAT, DBP and AGR2 are functionally implicated in the invasiveness of gastric cancer cells.
ISSN:	1016-8478 0219-1032
DOI:	10.1007/s10059-011-1053-z