DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex

Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumori...

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Veröffentlicht in:Molecules and cells 2016, 39(9), , pp.699-704
Hauptverfasser: Jang, Soo Hwa, Kim, Ah-Ram, Park, Neung-Hwa, Park, Jeong Woo, Han, In-Seob
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Sprache:eng
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Zusammenfassung:Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [(3)H] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but up-regulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.
ISSN:1016-8478
0219-1032
DOI:10.14348/molcells.2016.0149