포도 잿빛곰팡이병 저항성 판별에 영향하는 요인과 기내 검정방법

포도 잿빛곰팡이병원균에 대한 최적의 저항성 조건을 탐색하기 위해 ‘Thompson Seedless’, ‘Reliance’, ‘New York Muscat’, ‘Alden’, ‘Golden Muscat’, ‘Sheridan’, ‘Concord’, ‘Niagara’ 등 8개 품종을 사용하여 조사하였다. 효과적인 병저항성 검정을 위해 접종방법(상처, 무상처접종), 접종원(포자현탁액접종과 균체접종), 온도조건(15, 20, 25℃), 그리고 영양원 등의 조건을 시험하여 과방에서의 저항성 정도와 비교하였다. 그 결과 통계적인 유의성을 나타낸 온도조건 20℃와 엽령조건 중엽에 상처를 내지 않고 영양원으로서 PD broth를 24mg･mL-1 첨가한 포자현탁액을 엽 뒷면에 접종한 후 6일 뒤 조사하였을 때 포도 품종간의 저항성 정도를 분명하게 확인할 수 있었다. 본 저항성 검정 조건을 이용하여 유전자원 80종을 저항성 검정한 결과 ‘Thompson SDS’, ‘Pione’, ‘Ruby Okuyama’ 등이 감수성으로 나타났고 ‘Muscat Hamburg’, ‘Fujiminori’, ‘Steuben’ 등은 저항성으로 밝혀졌다. The optimal screening condition for evaluating resistance against inoculation of gray mold (Botrytis cinerea) was investigated on 8 different grapevines (Vitis spp.) varieties such as ‘Thompson Seedless’, ‘Reliance’, ‘New York Muscat’, ‘Alden’, ‘Golden Muscat’, ‘Sheridan’, ‘Concord’, and ‘Niagara’. The efficient screening system including inoculation method (spore suspension application and agar block inoculation), temperature (15, 20, and 25℃), and nutrient sources was developed and compared with the results obtained from other previous researches. As results, the factors temperature (20℃) and leaf age were included in the best model. Moreover, inoculating unwounded grapevine leaf of middle stage with spore suspension (106 spores･mL-1), prepared with 24 mg･mL-1 of potato dextrose broth (PDB), showed clear differences among various grape varieties. After inoculation the vines maintained in saturated humidity at 20℃ showed different levels of resistance to gray mold among various grape cultivars. This screening system may be very useful for the precise evaluation of the resistance to gray mold by pathogen inoculation in the grape breeding programs. KCI Citation Count: 4