One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity

The rising cases of multidrug-resistant ( ) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/ system, we constructed a stable, selectable marker-free autoluminescent capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti- drug candidates in vitro.