소 태아 혈청이 연골의 Proteoglycan 합성에 미치는 영향

Articular cartilage is a highly specialized connective tissue that covers the ends of long bones within the synovial joint cavity. It contains no blood vessels, no nerve fibers, and no lymphatics, accordingly it is nourished from the synocial flued by diffusion. Articular cartilage is composed of an...

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Veröffentlicht in:Kosin Medical Journal (Online) 1994, 10(1), , pp.41-47
Hauptverfasser: 김흥준, 김재도, 김영옥, 허만하
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Sprache:kor
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Zusammenfassung:Articular cartilage is a highly specialized connective tissue that covers the ends of long bones within the synovial joint cavity. It contains no blood vessels, no nerve fibers, and no lymphatics, accordingly it is nourished from the synocial flued by diffusion. Articular cartilage is composed of and extracellular matrix(ECM), collagen and proteoglycan. A small number of specific cells, chondrocytes, are embedded in the matrix. They are influenced by the alteration of components in synovial fluid which is derived from serum. To examine the effect of serum on biosynthesis of proteoglycan, this experiment was performed using human articular cartilages which were obtained from the patients who were operated due to various diseases. The human articular cartilages were incubated in 3 kinds of media : DMEM without fetal calf serum(FCS) (control), DMEM containing 1% FCS, and DMEM containing 20% FCS. The synthethized proteoglycan, incorporated with labeled S³⁵ sulfate, was extracted through PD-10 column after digestion by papain and measured the uptake of [35S] sulfate labeled proteoglycan by scintillation counter. To examine whether difference in concentration of fetal calf serum is reflected in staining intensity of immunohistochemistry for proteoglycan(avidin-biotin peroxidase complex), immunohistochemical staining was performed. The results were as followings : 1) Biosyntheses of proteoglycan in 1% and 20% FCS were increased 1.77 times and 2.27 respectively comparing with the control group. 2) In FCS group, biosynthesis of proteoglycan in 20% FCS was 1.29 times higher than that in 1% FCS. 3) No difference in staining intensity for proteoglycan in the variable concentrations of fetal calf serum was seen. The above results suggest that the compositional alteration of synovial fluid due to various factors influence the synthesis of the ECM in the articular cartilage and immunohistochemical staining is not sensitive enough to differentiate the quantitative difference of antigen. KCI Citation Count: 0
ISSN:2005-9531
2586-7024