Synthetic Biology Tools for Novel Secondary Metabolite Discovery in Streptomyces

are attractive microbial cell factories that have industrial capability to produce a wide array of bioactive secondary metabolites. However, the genetic potential of the species has not been fully utilized because most of their secondary metabolite biosynthetic gene clusters (SM-BGCs) are silent under laboratory culture conditions. In an effort to activate SM-BGCs encoded in genomes, synthetic biology has emerged as a robust strategy to understand, design, and engineer the biosynthetic capability of secondary metabolites. In this regard, diverse synthetic biology tools have been developed for species with technical advances in DNA synthesis, sequencing, and editing. Here, we review recent progress in the development of synthetic biology tools for the production of novel secondary metabolites in , including genomic elements and genome engineering tools for , the heterologous gene expression strategy of designed biosynthetic gene clusters in the chassis strain, and future directions to expand diversity of novel secondary metabolites.