Tardibacter chloracetimidivorans gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from an agricultural soil from Jeju Island in Republic of Korea

A pale yellow bacterial strain, designated JJ-A5 T , was isolated form an agricultural soil from Jeju Island in Republic of Korea. Cells of the strain were Gram-stain-negative, motile, flagellated and rod-shaped. The strain grew at 15–30°C, pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Growth occurred on R2A, but not on Luria-Bertani agar, nutrient agar, trypticase soy agar and MacConkey agar. The strain utilized alachlor as a sole carbon source for growth. The strain JJ-A5 T showed 16S rRNA gene sequence similarities lower than 95.4% with members of the family Sphingomonadaceae . Phylogenetic analysis showed that the strain belongs to the family Sphingomonadaceae and strain JJ-A5 T was distinctly separated from established genera of this family. The strain contained Q-10 as dominant ubiquinone and spermidine as major polyamine. The predominant cellular fatty acids were summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ), summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ), 11-methyl C 18:1 ω 7 c , C 16:0 and C 14:0 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, and phosphatidylcholine. The DNA G + C content of the strain was 62.7 mol%. On the basis of the phenotypic, genomic and chemotaxonomic characteristics, strain JJ-A5 T is considered to represent a novel genus and species within the family Sphingomonadaceae , for which the name Tardibacter chloracetimidivorans gen. nov., sp. nov. is proposed. The type strain of Tardibacter chloracetimidivorans is JJ-A5 T (= KACC 19450 T = NBRC 113160 T ).