Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)

Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)

Protein disulfide isomerase (PDI) catalyzes the oxidation of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have d...

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Veröffentlicht in:International Journal of Industrial Entomology 2010, 21(2), , pp.151-155
Hauptverfasser: Kim, S.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Yun, E.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, S.R., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Park, S.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kang, S.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Lee, K.G., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kwon, O.Y., Chungnam National University, Daejeon, Republic of Korea, Goo, T.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
Format: Artikel
Sprache:eng
Schlagworte:
BOMBYX MORI
Protein Disulfide Isomerase
Stem Cell Factor
농학
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Zusammenfassung:Protein disulfide isomerase (PDI) catalyzes the oxidation of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.
ISSN:1598-3579
2586-4785