Liquid chromatographic separation of the enantiomers of N-fluorenylmethoxycarbonyl α-amino acids on a covalently bonded type polysaccharide-derived chiral stationary phase under fluorescence detection

The liquid chromatographic enantiomer separation of N-fluorenylmethoxycarbonyl (FMOC) α-amino acids wasperformed using a covalently immobilized CSP (Chiralpak IB) with cellulose tris(3,5-dimethylphenylcarbamate) as a chiralselector under fluorescence detection. Several mobile phases with acid additives were used to show the effect of thereversed mobile phase on the chromatographic parameters of the separation and resolution factors. In this study, weobserved that the enantioseparation of investigated FMOC α-amino acids on Chiralpak IB using 90% MeOH/water with10 mM methanesulfonic acid as an additive in the reversed mobile phase (α = 1.18 - 2.31; Rs = 1.20 - 9.08) was significantlygreater than that using other mobile phases including the conventional normal mobile condition. Owing to higher sensitivityand selectivity in fluorescence detection than in UV detection, this analytical method using the reversed phase mode isexpected to enlarge their application of enantiomer resolution. KCI Citation Count: 0