DNA 합성관련 효소 억제제 및 인삼 Saponin이 Chinese Hamster Ovary (CHO) 세포손상에 미치는 영향

-Abstract- The physiological activity of Adriamycin (ADR) is related to its ability to bind specifically with DNA by intercalation between adjacent base pairs of the double helical structure and ADR induce DNA damage, chromosome aberrations and sister chromatid exchange in mammalian cell as the inhibitors of DNA synthesis related enzyme such as the aphidicolin (APC), novobiocin (Novo) and ginseng saponin (GS) on the frequencies of chromosome aberration, sister chromatid exchanges (SCEs), we treated ADR in CHO cells and post-treated APC, Novo and GS. 1. CHO cell viability by ADR was 60% in 1ng/ml and 0% 1ug/ml concentration. 2. When we treated ADR, APC, Novo and GS separately, only ADR increased chromosome aberrations and SCEs frequency. In proportion to the concentration of ADR increased, the aberration frequency was also increased. The more the concentration of ADR went up, the better the frequency of aberration rose. 3. In the group treated with ADR+APC or ADR+Novo, the frequency of the chromosome aberration and sister chromatid exchange were higher than the group treated with ADR alone, but decreased by post-treated ginseng saponin. The group treated with ADR, Novo and GS inhibited SCEs frequency significantly among the groups. These results suggest that GS can inhibit chromosome aberration and sister chromatid exchange induced by ADR, APC or ADR and Novo. KCI Citation Count: 0