Chemical constituents from Viburnum fordiae Hance and their anti-inflammatory and antioxidant activities

Three new neolignans, fordianoles A-C ( 1–3 ), characterized as (7 S ,8 R )-4-hydroxy-3,3′,5′-trimethoxy-8′,9′-dinor-8,4′-oxyneolignan-7,7′,9-triol, (7 R ,8 R )-4-hydroxy-3,3′,5′-trimethoxy-8′,9′-dinor-8,4′-oxyneolignan-7,7′,9-triol, and (7 R ,8 R )-4-hydroxy-3,3′,5′-trimethoxy-8,4′-oxyneolignan-7,9,9′-triol-7′-one, together with an unusual γ -lactone, 3-(3,4-dihydroxyphenyl)-4-pentanolide ( 4 ), and twenty-five known compounds ( 5 – 29 ) were isolated from the aerial parts of Viburnum fordiae Hance. Their structures including absolute configurations were determined by spectroscopic and chemical methods. Among them, compounds 6 , 7 , 11–15 , 17–28 were isolated from the Viburnum genus for the first time. The anti-inflammatory and antioxidant activities of all compounds were evaluated in vitro. Compounds 15 , 19 , 20 and 29 showed significant inhibitory activity on NO production in RAW264.7 cells stimulated by LPS with IC 50 values ranging from 8.60 to 13.92 μM. Meanwhile, compounds 1 – 4 , 15 , 19 , 20 , 22 , 23 , 25 , 26 and 29 exhibited varying antioxidant activities through DPPH, ABTS free radical scavenging and FRAP assays.