Effect of glutamine on heat-shock protein beta 1 (HSPB1) expression during myogenic differentiation in bovine embryonic fibroblast cells

The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 ( HSPB1 ) expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on HSPB1 expression during myogenesis, we treated with glutamine in myogenic lineage...

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Veröffentlicht in:	Food science and biotechnology 2018, 27(3), , pp.829-835
Hauptverfasser:	Kim, Young-Shin, Lee, Jae-Sung, Lee, Yoonseok, Kim, Won-Seob, Peng, Dong-Qiao, Bae, Mun-Hee, Jo, Yong-Ho, Baik, Myunggi, Lee, Hong-Gu
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Chemistry and Materials Science Desmin Embryo fibroblasts Fibroblasts Food Science Gene expression Glutamine Heat shock proteins mRNA Muscles MyoD protein Myogenesis Myogenin Myotubes Nutrition Protein biosynthesis Protein synthesis Ribonucleic acid RNA RNA-mediated interference siRNA TOR protein 식품과학
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Beschreibung
Zusammenfassung:	The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 ( HSPB1 ) expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on HSPB1 expression during myogenesis, we treated with glutamine in myogenic lineage determinant (MyoD) over-expressed bovine embryonic fibroblast cells (BEFS-MyoD cells). Second, knockdown of HSPB1 using small interference RNA was performed to evaluate whether muscle development by glutamine is dependent on HSPB1 in BEFS-MyoD cells. As a result, glutamine promoted the mRNA level of HSPB1 , Myogenin , Desmin , and mTOR as well as myotube formation, and protein synthesis ( p
ISSN:	1226-7708 2092-6456
DOI:	10.1007/s10068-018-0309-1