웅담과 우황약침이 지방세포 대사에 미치는 영향

Objectives : The purpose of this study is to investigate the effects of Fel ursi and Bovis calculus pharmacopuncture on primary cultured preadipocyte, adipocytes and localized fat tissue. Methods : Diminish preadipocyte proliferation and lipogenesis do primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1㎎/㎖ Fel ursi and Bovis calculus pharmacopuncture. Porcine skin including fat tissue after treated Fel ursi and Bovis calculus pharmacopuncture by means of the dosage dependent variation are investigated the histologic changes after injection of these pharmacopuncture. Results : Following results were obtained from the preadipocyte proliferation and lipolysis of adipocyte and histologic investigation of fat tissue. 1. Fel ursi and Bovis calculus pharmacopuncture showed the effect of decreased preadipocyte proliferation on the high dosage(1㎎/㎖). 2. Fel ursi pharmacopuncture showed the effect of decreased the activity of glycerol-3-phosphate dehydrogenase (GPDH) on the high dosage(1㎎/㎖) and Bovis calculus pharmacopuncture significantly showed from 0.1㎎/㎖ concentration. 3. Fel ursi pharmacopuncture was not showed the effect of lipolysis, but Bovis calculus pharmacopunc-ture was increased the effect of lipolysis in all concentration significantly. 4. Investigated the histological changes in porcine fat tissue after treated Fel ursi and Bovis calculus pharmacopuncture, we knew that these pharmacopuncture was showed significant activity to the lysis of cell membranes in all concentration. Conclusion : These results suggest that Fel ursi and Bovis calculus pharmacopuncture efficiently induces diminish proliferation of preadipocyte and lipolysis in adipose tissue.Key words in context : Fel ursi, Bovis calculus, pharmacopuncture, preadipocyte, adipocytes, lipolysis KCI Citation Count: 12