Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of veterinary science (Suwŏn-si, Korea) 2012, 13(3), , pp.323-325
Hauptverfasser:	Luka, Pam Dachung, National Veterinary Research Institute, Plateau State, Nigeria, Ayebazibwe, Chrisostom, Ministry of Agriculture, Animal Industry and Fisheries, Entebbe, Uganda, Shamaki, David, National Veterinary Research Institute, Plateau State, Nigeria, Mwiine, Frank Norbert, Makerere University, Kampala, Uganda, Erume, Joseph, Makerere University, Kampala, Uganda
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CAPRIN CAPRINOS DIAGNOSIS DIAGNOSTIC DIAGNOSTICO DNA Primers - analysis Eye - virology Goat Diseases - blood Goat Diseases - diagnosis Goat Diseases - epidemiology Goat Diseases - virology GOATS Hair - virology Nose - virology Nucleoproteins - analysis OVIN OVINOS peste des petits ruminants Peste-des-Petits-Ruminants - blood Peste-des-Petits-Ruminants - diagnosis Peste-des-Petits-Ruminants - epidemiology Peste-des-Petits-Ruminants - virology Peste-des-petits-ruminants virus - genetics Peste-des-petits-ruminants virus - isolation & purification Pigmentation Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - standards Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Viral - genetics RNA, Viral - isolation & purification sample SHEEP Sheep Diseases - blood Sheep Diseases - diagnosis Sheep Diseases - epidemiology Sheep Diseases - virology Short Communication Uganda - epidemiology 수의학
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that bully coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
ISSN:	1229-845X 1976-555X
DOI:	10.4142/jvs.2012.13.3.323