Development of monoclonal antibodies against the abnormal prion protein isoform (PrP∨res) associated with chronic wasting disease (CWD)

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP∨res) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of t...

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Veröffentlicht in:	Journal of veterinary science (Suwŏn-si, Korea) 2012, 13(4), , pp.429-432
Hauptverfasser:	Jeong, H.J., Konkuk University, Seoul, Republic of Korea, Lee, N.H., Konkuk University, Seoul, Republic of Korea, Lee, J.B., Konkuk University, Seoul, Republic of Korea, Park, S.Y., Konkuk University, Seoul, Republic of Korea, Song, C.S., Konkuk University, Seoul, Republic of Korea, Seo, K.H., Konkuk University, Seoul, Republic of Korea, Kim, D.W., National Institute of Animal Science, RDA, Cheonan, Republic of Korea, Kim, Y.S., Ilsong Institute of Life Science, Hallym University, Anyang, Republic of Korea, Choi, I.S., Konkuk University, Seoul, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	ALCE Animals Antibodies, Monoclonal - genetics Blotting, Western - veterinary CWD Deer - immunology Deer - metabolism DNA Primers - genetics ELAN Electrophoresis, Polyacrylamide Gel - veterinary ELKS Endopeptidase K Mice Mice, Knockout monoclonal antibody Polymerase Chain Reaction - veterinary PRION PRIONES PRIONS Prions - genetics Prions - immunology Protein Isoforms - genetics Protein Isoforms - immunology rPres Short Communication Wasting Disease, Chronic - diagnosis Wasting Disease, Chronic - immunology 수의학
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Zusammenfassung:	Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP∨res) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
ISSN:	1229-845X 1976-555X
DOI:	10.4142/jvs.2012.13.4.429