Gossypol acetic acid induces apoptosis in RAW264.7 cells via a caspase-dependent mitochondrial signaling pathway

To investigate the effects of gossypol acetic acid on proliferation and apoptosis of the macrophage cell line RAW264.7 and Further understand the possile underlying mechanism responsible for GA-induced cell apoptosis, RAW 264.7 cells were treated with GA for 24h and the cytotoxicity was determined b...

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Veröffentlicht in:	Journal of veterinary science (Suwŏn-si, Korea) 2013, 14(3), , pp.281-289
Hauptverfasser:	Deng, S.J., Human Agricultural University, Changsha, China, Yuan, H., Human Agricultural University, Changsha, China, Yi, J., Human Agricultural University, Changsha, China, Lu, Y., Human Agricultural University, Changsha, China, Wei, Q., Human Agricultural University, Changsha, China, Guo, C.Z., Shanghai Jiao Tong University School of Medicine, Shanghai, China, Wu, J., Renji Hospital , Shanghai, China, Yuan, L., Renji Hospital , Shanghai, China, He, Z., Shanghai Jiao Tong University School of Medicine, Shanghai, China
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antineoplastic Agents, Phytogenic - pharmacology APOPTOSE APOPTOSIS Apoptosis - drug effects Cell Line Cell Proliferation - drug effects Dose-Response Relationship, Drug GOSIPOL GOSSYPOL Gossypol - analogs & derivatives Gossypol - pharmacology Membrane Potential, Mitochondrial - drug effects Mice Mice, Inbred BALB C mitochondrial signaling pathway Original Reactive Oxygen Species - metabolism Signal Transduction - drug effects 수의학
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Zusammenfassung:	To investigate the effects of gossypol acetic acid on proliferation and apoptosis of the macrophage cell line RAW264.7 and Further understand the possile underlying mechanism responsible for GA-induced cell apoptosis, RAW 264.7 cells were treated with GA for 24h and the cytotoxicity was determined by MTT assay, while apoptotic cells were identified by TUNEL assay, acridine orange/ethidium bromide standing and flow cytometry.
ISSN:	1229-845X 1976-555X
DOI:	10.4142/jvs.2013.14.3.281