국내 기장 및 수수의 바이러스병 발생 조사
Throughout year 2015 to 2016, 101 proso millet and 200 sorghum samples were collected from five provinces in South Korea. The samples were subjected to paired-end RNA sequencing and further analyzed by RT-PCR. The results indicated that Rice black-streaked dwarf virus (RBSDV) was detected from sorgh...
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Veröffentlicht in: | Sigmulbyeong yeon'gu 2017, 23(3), , pp.262-267 |
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Sprache: | kor |
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Zusammenfassung: | Throughout year 2015 to 2016, 101 proso millet and 200 sorghum samples were collected from five provinces in South Korea. The samples were subjected to paired-end RNA sequencing and further analyzed by RT-PCR. The results indicated that Rice black-streaked dwarf virus (RBSDV) was detected from sorghum collected in Gyeongsang province. The other four viruses, including RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), and Cereal yellow dwarf virus (CYDV), were detected from proso millet. Among four viruses, both RSV and RBSDV were identified high frequency from proso millet collected from Gyeongsang province. Otherwise, BVG was nearly equally identified from five provinces, suggesting that the virus was supposedly widespread nationwide. RBSDV was first identified from both proso millet and sorghum in South Korea. The other virus annotated CYDV identified proso millet was shown to have relatively low identities compared to CYDV previously reported, suggesting that the virus might be new member of Polerovirus. 2015년부터 2016년까지 국내 5개 지역에서 기장 101점, 수수 200점의 시료를 채집하였다. 채집한 시료에 대해 paired-end RNA sequencing, RT-PCR 진단을 수행하였다. 그 결과, 경상도에서 채집한 수수에서 Rice black-streaked dwarf virus (RBSDV)가 검출되었으며, RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), Cereal yellow dwarf virus (CYDV) 4종의 바이러스가 기장에서 검출되었다. 기장에서 검출된 4종의 바이러스 중 RSV와 RBSDV는 경상도에서 채집한 시료에서 높은 감염률을 보였다. 반면, BVG는 5개 지역에서 모두 감염이 확인되었으며, 이미 전국적으로 분포되어 있는 바이러스인 것으로 보인다. 본 연구에서는 국내 기장과 수수에서 RBSDV를 처음으로 동정하였으며, 수수에서 검출된 CYDV는 기존에 보고된 CYDV 분리주와 상동성이 상대적으로 낮은 것으로 보아 Polerovirus 속의 신종 바이러스로 예상된다. |
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ISSN: | 1598-2262 2233-9191 |