NanoString nCounter® Approach in Breast Cancer: A Comparative Analysis with Quantitative Real-Time Polymerase Chain Reaction, In Situ Hybridization, and Immunohistochemistry
Accurate testing for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) is essential for breast cancer treatment. At present, immunohistochemistry (IHC)/florescence hybridization (FISH) are widely accepted as the standard testing methods. To inves...
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Veröffentlicht in: | Journal of breast cancer 2017, 20(3), 83, pp.286-296 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Accurate testing for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) is essential for breast cancer treatment. At present, immunohistochemistry (IHC)/florescence
hybridization (FISH) are widely accepted as the standard testing methods. To investigate the value of NanoString nCounter®, we performed its comparative analysis with IHC/FISH and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) for the assessment of ER, PR, and HER2.
Data on IHC/FISH results for ER, PR, and HER2 in 240 patients from a single tertiary hospital in Korea were collected and compared with NanoString nCounter® and qRT-PCR results at a single institution.
Expression levels for each gene using NanoString nCounter® showed good correlation with the corresponding data for protein expression by IHC ( |
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ISSN: | 1738-6756 2092-9900 |
DOI: | 10.4048/jbc.2017.20.3.286 |