Epigallocatechin gallate attenuates L-DOPA-induced apoptosis in rat PC12 cells

In this study, the protective effects of EGCG on L-3,4-dihydroxyphenylalanine (L-DOPA)-induced oxidative cell death in catecholaminergic PC12 cells, the in vitro model of Parkinson’s disease, were investigated. Treatment with L-DOPA at concentrations higher than 150 μM caused cytotoxicity in PC12 ce...

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Veröffentlicht in:Nutrition research and practice 2013, 7(4), , pp.249-255
Hauptverfasser: Lee, M.Y., Chosun University, Gwangju, Republic of Korea, Choi, E.J., Chosun University, Gwangju, Republic of Korea, Lee, M.K., Chungbuk National University, Cheongju, Republic of Korea, Lee, J.J., Chosun University, Gwangju, Republic of Korea
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Sprache:eng
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Zusammenfassung:In this study, the protective effects of EGCG on L-3,4-dihydroxyphenylalanine (L-DOPA)-induced oxidative cell death in catecholaminergic PC12 cells, the in vitro model of Parkinson’s disease, were investigated. Treatment with L-DOPA at concentrations higher than 150 μM caused cytotoxicity in PC12 cells, as determined using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry detection. The apoptotic ratio was similar in cells treated with 100 μM EGCG plus 150 μM L-DOPA (5.02%) and the control (0.96%) (P 0.05), and was lower than that of cells treated with L-DOPA only (32.24%, P 0.05). The generation level of ROS (% of control) in cells treated with EGCG plus L-DOPA was lower than that in cells treated with L-DOPA only (123.90% vs 272.32%, P 0.05). The optical density in production of TBARS in cells treated with L-DOPA only was higher than that in the control (0.27 ± 0.05 vs 0.08 ± 0.04, P 0.05), and in cells treated with EGCG only (0.14 ± 0.02, P 0.05), and EGCG plus L-DOPA (0.13 ± 0.02, P 0.05). The intracellular level of GSH in cells treated with EGCG plus L-DOPA was higher than that in cells treated with L-DOPA only (233.25 ± 16.44 vs 119.23 ± 10.25, P 0.05). These results suggest that EGCG protects against L-DOPA-induced oxidative apoptosis in PC12 cells, and might be a potent neuroprotective agent.
ISSN:1976-1457
2005-6168
DOI:10.4162/nrp.2013.7.4.249