Callus induction and plant regeneration of Lychnis wilfordii (Regel) Maxim a critically endangered plant in Korea

Lychnis wilfordii (Regel) Maxim is a rare andvalued ornamental plant. Germination rate reached 46.6%when seeds were treated with 100 mg‧l-1 GA (Gibberellicacid)3. The highest callus induction was observed in the leafexplants of the seedlings on MS medium containing specificconcentrations of 0.5 mg‧l-1 BA (N6-benzyladenine) and 3.0mg‧l-1 NAA (a-naphthalene acetic acid). The adventitiousshoot was formed in 97.3% of calli on 1/2 WPM (Woody PlantMedium) medium. Shoot elongation of in vitro propagatedplantlets was no difference among various medium. Theplantlets grew well after transferring to the pot. This in vitropropagation protocol should be useful for conservation ofthis endangered plant. KCI Citation Count: 1