Cultural Characteristics of Xanthomonas axonopodis pv.citri Bacteriophages CP1 from Korea

Bacteriophage of Xantomonas axonopodis pv. citri, a causal agent of citrus canker disease, was studied for its cultural characteristics. The relative efficiency of plating (EOP) of 11 phages used to 13 strains of X. axonopodis pv. citri tested ranged from 0.8 to 1, indicating that the phages are homogeneous. Homogeneity of the phages suggests that citrusphage belongs to a single group CPK as reported in a previous study. Typical one-step growth of a phage P5 selected from the citrusphages was observed. The EOP of the P5 was dependent upon the media, pH, and temperature. It was observed that multiplication of the phage cultured in Wakimotos potato semisynthetic media at 25oC was more effective than that in other temperatures, regardless of the bacterial strains and media used. It was observed that pH 6.5 is optimal for multiplication of the phage. In comparison of the EOP among citrusphages CP1, CP2, and P5, multiplicative characteristic of phage P5 in the bacteria on time-course was similar with that of phage CP1. Thus, it was concluded that citrusphage group CPK from Korea is CP1 based on host specificity of the phage as described in a previous study, homogeneity, and its multiplication pattern. KCI Citation Count: 0