Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina

Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina

This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 a...

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Veröffentlicht in:The plant pathology journal 2010, 26(3), , pp.289-292
Hauptverfasser: Kim, J.S., The University of Seoul, Seoul, Republic of Korea, Seo, S.G., The University of Seoul, Seoul, Republic of Korea, Jun, B.K., The University of Seoul, Seoul, Republic of Korea, Kim, J.W., The University of Seoul, Seoul, Republic of Korea, Kim, S.H., The University of Seoul, Seoul, Republic of Korea
Format: Artikel
Sprache:eng
Schlagworte:
CERCOSPORA SOJINA
DNA extraction
fungus
RAPD
Restriction enzyme
농학
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Beschreibung
Zusammenfassung:This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis.
ISSN:1598-2254
2093-9280
DOI:10.5423/PPJ.2010.26.3.289