Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis

spp. are contagious bacteria, and mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of on bMECs has not been fully described. To elucidate the immune response against intramammary infection by , we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with . We also compared the effects of , and on immune-related mRNA expression in bMECs. Transcriptome analysis indicated a significant decrease in the level of mRNA-encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL)-1β, IL-6, tumor necrosis factor alpha, toll-like receptor (TLR) 2, and TLR4 mRNA expression levels were significantly increased in bMECs stimulated with heat-killed , but the expression levels were lower than those following stimulation by heat-killed or . Our results suggest that weakly affects mRNA expression in bMECs compared to the effects of or . Moreover, live may induce suppression of the immune response in bMECs.