Anti-Inflammatory Effects of Hexane Fraction from White Rose Flower Extracts via Inhibition of Inflammatory Repertoires

In this study, we determined the anti-infl ammatory activity and mechanism of action of a hexane fraction (hWRF) obtained from white Rosa hybrida fl owers by employing various assays such as quantitative real-time PCR, Western blotting, and Electrophoretic-Mobility Shift Assay (EMSA). The results revealed that the hWRF had excellent anti-infl ammatory potency by reducing infl ammatory repertoires, such as inducible nitric oxide synthase (iNOS), interleukin-1β, and cyclooxygenase-2 (COX-2) in RAW264.7cells when stimulated with lipopolysaccharide (LPS), a pro-infl ammatory mediator. The reduction of nitric oxide (NO) release from RAW 264.7 cells supported the anti-infl ammatory effect of hWRF. Interestingly, hWRF effectively inhibited LPS-mediated nuclear factor-κB (NF-κB) p65 subunit translocation into the nucleus and extracellular signal-regulated kinase (ERK)1/2 phosphorylation,suggesting that hWRF anti-infl ammatory activity may be based on inhibition of the NF-κB and MAPK pathways. Based on the fi ndings described in this study, hWRF holds promise for use as a potential anti-infl ammatory agent for either therapeutic or functional adjuvant purposes. KCI Citation Count: 10