유기물 장기 연용이 밭토양 미생물의 다양성에 미치는 영향

To investigate the effect of long term application of organic matter in upland soils, plots for treatments of NPK, NPK+pig manure compost, rape seed cake, rice straw compost, and green manure were set up. Populations of Bacillus and Gram negative bacteria were high in the plot treated with green manure application, but microbial biomass was increased with chemical fertilizer or pig manure compost in upland soils. Activities of phosphomonoesterase and dehydrogenase were high with organic matter application comparing to control. Cluster patterns analysed using phospholipid fatty acid of plots treated with rice straw and or pig manure compost were clearly different comparing with other treatments. Dominant bacteria in upland soils were Bacillus flexus, B. subtilis and B. megaterium. And the strains isolated from upland soils had amylase, protease and lipase activities. 바실러스와 그람음성균은 녹비시용구에서 높았지만, 미생물량은 비료+돈분퇴비 시용구에서 높았다. 인산효소와 유기물분해효소는 무처리구에 비해 유기물 시용구에서 모두 높은 값을 보였다. 영년 밭토양의 PLFA에 의한 군집분석은 볏짚퇴비 및 돈분퇴비시용구가 다른 처리와 상이한 특성을 보였다. 영년 밭 토양의 우점균은 Bacillus flexus, Bacillus subtilis 및 Bacillus megaterium 등이었으며, 토양에서 분리된 세균은 대부분 amylase, protease 및 lipase의 활성을 가지고 있었다.