Continuous Cultivation of Lactobacillus rhamnosus with Cell Recycling Using an Acoustic Cell Settler

Continuous production of lactic acid from glucose by Lactobacillus rhamnosus with cellconcentration of cell and product were compared with the control experiment without recycling. The acoustic setler showed cel separation efficiency of 67. during the continuous operation and the cel concentration in the fermentor with recycle exceeded that of the control by 29%. Compared with the control, lactic acid production was increased by 40%, while glucose consumption was only increased by 8%. The higher value of lactic acid production to substrate consumption (YP/Scled cel mass consumes les substrate to produce the same amount of product than the control. Within system environmental changes due to the longer mean cel residence time induced the cels maintaining the metabolic pathways to produce less by-product but more product, lactic acid. KCI Citation Count: 0