Phellinus Extracts Inhibit Migration and Matrix Metalloproteinase Secretion in Porcine Coronary Artery Endothelial Cells

Phellinus linteus is a fungus which is found primarily in tropical regions of the Americas, Africa, and Asia. P. linteus has been used in traditional medical practice for the treatment of arthritis, liver damage and cancer. Angiogenesis is a process that involves migration, proliferation and cell differentiation, as well as the formation of new capillary structures. The antiangiogenic activities evidenced by natural compounds may actually be a critical effect for the inhibition of angiogenesis-dependent disease by these agents via the blockage of vascular development. This study assessed the effects of water extracts from P. linteus (Phellinus extracts) on primary cultured porcine coronary artery endothelial cells (PCAECs). Phellinus extracts induced no changes in DNA synthesis or cell numbers, but inhibited the migration of PCAECs. Phellinus extracts also induced a reduction in the secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9. Our results show that, in endothelial cells, Phellinus extracts may inhibit angiogenesis by reducing levels of MMP-2 and MMP-9 secretion.