Simultaneous Extraction and Separation of Liquiritin, Glycyrrhizic Acid, and Glabridin from Licorice Root with Analytical and Preparative Chromatography
Simultaneous extraction and separation of liquiritin, glycyrrhizic acid, and glabridin from licorice were developed by liquid-liquid extraction with liquid chromatography separation. By utilizing different extraction solvents, procedures, and times, the optimum extraction conditions were established...
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Veröffentlicht in: | Biotechnology and bioprocess engineering 2008, 13(6), , pp.671-676 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Simultaneous extraction and separation of liquiritin, glycyrrhizic acid, and glabridin from licorice were developed by liquid-liquid extraction with liquid chromatography separation. By utilizing different extraction solvents, procedures, and times, the optimum extraction conditions were established. The extracts of licorice were separated and determined using a C∧18 column with a mobile phase consisting of acetonitrile-water (containing 1.0% acetic acid) with a gradient elution of 0~10 min from 20:80 to 60:40 (v/v). Preparative columns with different packing sizes were investigated to isolate the three compounds from the extracts of licorice. The 12 ㎛ chromatographic column showed better separation for the three compounds from licorice. 0.29 mg/g for liquiritin, 1.43 mg/g for glycyrrhizic acid, and 0.07 mg/g for glabridin were obtained and the recoveries were 80.8, 89.7, and 72.5%, respectively. |
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ISSN: | 1226-8372 1976-3816 |
DOI: | 10.1007/s12257-008-0019-2 |