Optimization of APE1547-catalyzed Enantioselective Transesterification of (R/S)-2-methyl-1-butanol in an Ionic Liquid

Optimization of APE1547-catalyzed Enantioselective Transesterification of (R/S)-2-methyl-1-butanol in an Ionic Liquid

Aeropyrum pernix esterase (APE 1547) was successfully used to catalyze the enantioselective transesterification of (R/S)-2-methyl-1-butanol in an ionic liquid (IL). Effects of various reaction conditions on the synthetic activity of the enzyme as well as enantioselectivity, including the type of IL,...

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Veröffentlicht in:Biotechnology and bioprocess engineering 2011, 16(2), , pp.337-342
Hauptverfasser: Tian, Rui, The Second Hospital of Jilin University, Changchun, China, Yang, Chun-Hua, Changchun Medical College, Changchun, China, Wei, Xiao-Fei, Jilin University, Changchun, China, Xun, Er-Na, Jilin University, Changchun, China, Wang, Ren, Jilin University, Changchun, China, Cao, Shu-Gui, Jilin University, Changchun, China, Wang, Zhi, Jilin University, Changchun, China, Wang, Lei, Jilin University, Changchun, China
Format: Artikel
Sprache:eng
Schlagworte:
2-methyl-1-butanol
Aeropyrum pernix
Aeropyrum pernix esterase (APE1547)
Biotechnology
Catalysis
catalytic activity
Chemistry
Chemistry and Materials Science
Chromatography
enantioselectivity
enzyme activity
Enzymes
ESTERASAS
ESTERASE
ESTERASES
High temperature
Industrial and Production Engineering
ionic liquid
ionic liquids
Optimization
Research Paper
Sensors
Solvents
Studies
temperature
transesterification
Viscosity
water activity
생물공학
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Zusammenfassung:Aeropyrum pernix esterase (APE 1547) was successfully used to catalyze the enantioselective transesterification of (R/S)-2-methyl-1-butanol in an ionic liquid (IL). Effects of various reaction conditions on the synthetic activity of the enzyme as well as enantioselectivity, including the type of IL, acyl donor, temperature, water activity, and substrate molar ratio were inverstigated. APE1547 showed good catalytic performance (activity greater than 0.8 μmol/min/mg, E greater than 25), and the enzyme-IL mixture was recycled five times with only a slight decrease in catalytic performance.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-010-0253-2